α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle Type III, ammonium sulfate suspension, TPI 750-2000 units/mg protein, GDH 75-200 units/mg protein (biuret) Sigma G1881
Packaging
(Sold on the basis of α-GDH units.)
Unit Definition
(α-GDH) One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.
(TPI) One unit will convert 1.0 μmole of
Physical form
Mixed crystalline suspension in 3.2 M (NH4)2SO4, pH 6
Application
α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.1
Biochem/physiol Actions
α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate2.
Характеристики реактива | |
storage temp. | 2-8°C |