α-Galactosidase I, Alkaline from Cucumis melo Sigma G7673
По запросу
- Производитель: Sigma
- Каталожный номер:
G7673 - CAS номер : 9025-35-8
Biochem/physiol Actions
α-Galactosidase form I from melon may play a significant role in photoassimilate partitioning in sink tissue. The form I enzyme has a preferred activity with raffinose and significant activity with stachyose. A unique feature of this enzyme is that it exhibits weak product inhibition by galactose.
This enzyme shows a preference for raffinose and also activity with stachyose. Unlike other a-Galctosidases, this enyzme also shows weak inhibition by galactose.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-
Physical form
The product is supplied as a lyophilized powder containing Tris-HCl buffer salts, DTT, EDTA, and NaCl.
Application
Alkaline α-Galactosidase I was used to assay enzyme activity with 2 mmp-nitrophenyl-α-d-galac
General description
Alkaline α-Galactosidase I is a glycoside hydrolase, separated from the extracts of melon fruits. The activity of this enzyme is found to increase during the early stages of ovary development and fruit set.2
Physical properties
This enzyme exhibits maximal activity between 30-37°C. Activity declines above 40°C.
Характеристики реактива | |
assay | ≥85% (SDS-PAGE) |
storage temp. | −20°C |