α-Chymotrypsinogen A from bovine pancreas essentially salt-free, lyophilized powder Sigma C4879
По запросу
- Производитель: Sigma
- Каталожный номер:
C4879 - CAS номер : 9035-75-0
Biochem/physiol Actions
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.
Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges and requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form α, β, γ , δ, and π chymotrypsin (depending upon the conditions of activation). Its pI is 8.97.
Other Notes
View more information on chymotrypsin at www.sigma-aldrich.com/enz
Packaging
1, 5 g in poly bottle
100, 250 mg in poly bottle
Preparation Note
Derived from New Zealand-sourced pancreas.
Unit Definition
After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
Application
The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy.1 It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems.2 The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.3
Характеристики реактива | |
storage temp. | −20°C |