α-Chymotrypsin from bovine pancreas suitable for protein sequencing, salt-free, lyophilized powder Sigma C6423
По запросу
- Производитель: Sigma
- Каталожный номер:
C6423 - CAS номер : 9004-07-3
Biochem/physiol Actions
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
Other Notes
View more information on chymotrypsin at www.sigma-aldrich.com/enz
Unit Definition
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
Application
α-Chymotrypsin from bovine pancreas is used for the following applications:
• Protein Identification by mass spectrometry (MS)1
• The isolation and characterization of myosin heavy chains2
• Toxin preparation3
• The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE4
α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.5
General description
α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein.6 The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.7
Характеристики реактива | |
storage temp. | 2-8°C |