α-Actinin from chicken gizzard ~80% α-actinin basis (SDS-PAGE), ammonium sulfate suspension Sigma A9776
Cross-linking protein in the actin cytoskeleton
5 mg in poly bottle
Package size based on protein content
Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.
Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).
Suspension in 2 M (NH4)2SO4 containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride
|assay||~80% α-actinin basis (SDS-PAGE)|