β-Amylase from sweet potato Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280 ) Sigma A7005
По запросу
- Производитель: Sigma
- Каталожный номер:
A7005 - CAS номер : 9000-91-3
General description
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz
Packaging
100000 units in glass bottle
Unit Definition
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.
Physical form
Crystalline suspension in 2.3 M (NH4)2SO4
Application
β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides.1 β-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms.2 The enzyme has also been used to prepare β-limit dextrin from waxy maize starch.3
Biochem/physiol Actions
β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. β-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry.4
Характеристики реактива | |
storage temp. | 2-8°C |